Journal: PLoS ONE

Article Title: Circulating Endothelial Progenitor Cells in Kidney Transplant Patients

doi: 10.1371/journal.pone.0024046

Figure Lengend Snippet: Progenitor cells were defined by the surface expression of stem cell antigen-1 (Sca-1) and c-Kit antigens. The number of circulating progenitor cells (A) and CD26 + cells (B) was determined by flow cytometry 14 days after surgery/treatment. Sham: sham animals; 5/6 Nx: nephrectomy; CNI: calcineurin inhibitor (cyclosporine A 5 mg/kg/day); CNI free: mycophenolate mofetil 30 mg/kg/day. Results are mean ± SEM. * P<0.05 compared to Sham; # P<0.05 compared to vehicle.

Article Snippet: Briefly, 100 µl of EDTA-blood samples obtained from the tail vein were incubated at 4°C for 30 minutes in the dark with the following antibody combinations: 1) IgG2b-PE+IgG1-FITC; 2) PE-conjugated anti-mouse Sca-1 (Cederlane, Canada)+FITC-conjugated anti-mouse c-Kit (BD Pharmingen); 3) PE-conjugated anti-mouse Sca-1 (Cederlane, Canada)+biotin-conjugated anti-rat CD31 (BD Pharmingen)+Streptavidin-FITC (BD Pharmingen); and 4) PE-conjugated anti-rat CD26 antibody (BD Pharmingen).

Techniques: Expressing, Flow Cytometry

Journal: International Journal of Molecular Sciences

Article Title: Human Wharton’s Jelly-Derived Mesenchymal Stem Cells Minimally Improve the Growth Kinetics and Cardiomyocyte Differentiation of Aged Murine Cardiac c-kit Cells in In Vitro without Rejuvenating Effect

doi: 10.3390/ijms20225519

Figure Lengend Snippet: List of antibodies for CC characterisation and differentiation.

Article Snippet: FITC Rat Anti-mouse Sca-1 Antibody (Clone D7) , 1:10 , FC , Miltenyi Biotec, Germany (130-102-297).

Techniques: